Protocol for Using the Isolera-Biotage for "Wide-Polarity-Range" (WPR) v1
This protocol describes the procedure for fractionating complex mixtures using the Wide-Range-Polarity (WRP) method on the Isolera-Biotage system. This method employs a controlled elution gradient to separate compounds with a wide range of polarity, such as secondary metabolites from natural extracts. The method is applicable to silica gel or C18 stationary phase columns, depending on the sample characteristics.
Authors
- Rômulo Pereira de Jesus (ORCID: https://orcid.org/0000-0003-1490-2112)
- Ricardo M. Borges (ORCID: https://orcid.org/0000-0002-7662-6734)
Publication Details
- Published
- 2025-08-15
- DOI
- https://doi.org/10.17504/protocols.io.eq2ly4jjelx9/v1
- Primary Topic
- bioluminescence and chemiluminescence research
- Type
- article
- Field-Weighted Citation Impact
- 0.00