Isolation and identification of a new porcine astrovirus 5 demonstrated that oxidative stress enhances porcine astrovirus replication
Our study successfully isolated a novel strain of PAstV5 characterized by an 11-amino acid deletion in the nsp1a protein, leading to significant alterations in the three-dimensional structure of the nsp1a/4 protein. This strain was observed to induce the production of mitochondrial ROS, downregulate the expression of Nrf2 and HO-1, and cause mitochondrial damage. Furthermore, the generation of mitochondrial ROS was found to facilitate the replication of PAstV. These findings offer valuable insights into the genetic evolution and pathogenic mechanisms of PAstV.
Authors
- Weijian Huang (ORCID: https://orcid.org/0000-0003-2958-134X)
- Ying Chen
- Shaodong Song (ORCID: https://orcid.org/0009-0009-5374-2562)
- Kang Ouyang (ORCID: https://orcid.org/0000-0003-2082-2362)
- Xiaofang Wei (ORCID: https://orcid.org/0000-0003-1588-8797)
- Yifeng Qin (ORCID: https://orcid.org/0000-0002-2486-6154)
- Yanmin Lv (ORCID: https://orcid.org/0000-0002-1679-9174)
- Zuzhang Wei (ORCID: https://orcid.org/0000-0002-6317-2315)
- Yuhang Luo
- Yibin Qin (ORCID: https://orcid.org/0000-0002-8484-0968)
- Yeshi Yin (ORCID: https://orcid.org/0000-0001-6866-5525)
- Yiyang Du
- Xiaoying Feng
Institutions
- Guangxi University (CN)
- China Animal Disease Control Center (CN)
- Guangxi Veterinary Research Institute (CN)
- Guangxi Center for Disease Prevention and Control (CN)
- Ministry of Agriculture and Rural Affairs (CN)
Publication Details
- Journal
- BMC Veterinary Research
- Published
- 2025-08-19
- DOI
- https://doi.org/10.1186/s12917-025-04939-x
- Citations
- 1
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 1.40
Funders
- Natural Science Foundation of Guangxi Province
- Specific Research Project of Guangxi for Research Bases and Talents