A <sup>19</sup>F‐ATCUN Peptide NMR Probe for Selective Cu<sup>2+</sup> or Ni<sup>2</sup><sup>+</sup> Sensing in Complex Biological Media
We report the design and synthesis of ratiometric <sup>19</sup>F-ATCUN (Xxx-Zzz-His) peptide probes for the selective and reversible detection of Ni<sup>2</sup>⁺ and Cu<sup>2</sup>⁺ by <sup>19</sup>F NMR spectroscopy. In contrast to fluorescence-based approaches, <sup>19</sup>F NMR enables background-free detection in biological media, due to the absence of fluorine in native biomolecules. The probes remain stable and functional in complex cell media (DMEM), enabling the detection of Cu<sup>2</sup>⁺ and Ni<sup>2</sup>⁺ by <sup>19</sup>F NMR with a limit of detection (LOD) of 4 µM and quantification (LOQ) of 10 µM, relevant for studying copper-related pathologies.
Authors
- Laurent Raibaut (ORCID: https://orcid.org/0000-0002-8757-8379)
- Enrico Falcone (ORCID: https://orcid.org/0000-0002-1944-3552)
- Marine Desage‐El Murr (ORCID: https://orcid.org/0000-0001-5017-4496)
- Peter Faller (ORCID: https://orcid.org/0000-0001-8013-0806)
- Yannis‐Nicolas François (ORCID: https://orcid.org/0000-0002-8776-071X)
- Bruno Kieffer (ORCID: https://orcid.org/0000-0002-2033-4679)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Institut de génétique et de biologie moléculaire et cellulaire (FR)
- Institut de Chimie de Strasbourg (FR)
- Laboratoire de Chimie de Coordination (FR)
Publication Details
- Journal
- Chemistry - A European Journal
- Published
- 2025-08-19
- DOI
- https://doi.org/10.1002/chem.202501784
- Primary Topic
- Molecular Sensors and Ion Detection
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- China Scholarship Council