Native Whey as Raw Material for Biologically Active Peptides

Bioactive peptides come from various plant and animal protein sources, e.g., native whey. Native whey is a permeate obtained in the process of skim milk microfiltration. This research involved native whey obtained at the Kalacheevsky Cheese Factory, Voronezh Dairy Plant. Its composition was studied using standard methods, including mathematical statistics, to compare it with the composition of cheese whey. The protein and mineral profile of the native whey appeared to depend on the microfiltration conditions during processing. To obtain a protein isolate based on native whey, it was subjected to reverse osmosis concentration followed by ultrafiltration. The resulting native whey isolate had a protein mass fraction of 90.8±0.7% and served as a substrate for enzymatic hydrolysis, which involved two different enzyme preparations, Protamex and Flavourzyme. The hydrolysis degree was 35%. The research confirmed the feasibility of using native whey as a raw material for biologically active peptides.