Rapid and simple detection of Listeria monocytogenes using real closed dumbbell-mediated isothermal amplification

The real-time fluorescence plots and following melting curve analysis-based <i>L. monocytogenes</i> O-CDA were suitable for laboratory-based diagnosis. Considering the portable manipulation of the HNB-based colorimetric detection system, our results shed light on its potential application for on-site <i>L. monocytogenes</i> surveillance. The developed CDA-based methods are rapid, simple, reliable, and sensitive in several samples, showing potential to manage the task of <i>L. monocytogenes</i> monitoring more easily.