Identification of anti-fibrotic compounds from Piper longum L via hollow fiber cell fishing and high-performance liquid chromatography with in vivo and in vitro validation
A novel HFCF-HPLC model was successfully established to screen active compounds from TCM against renal fibrosis. PIPA was identified as a promising anti-fibrotic agent from Piper longum L, demonstrating significant renoprotective effects in in vitro and in vivo models. This work advances the modernization of herbal medicine research by offering a integrated strategy for identifying bioactive TCM components.
Authors
- Wanghui Jing (ORCID: https://orcid.org/0000-0001-9594-2989)
- Guoping Zheng (ORCID: https://orcid.org/0000-0002-5551-4522)
- Heng Wang (ORCID: https://orcid.org/0000-0001-7408-0398)
- Jie Tian (ORCID: https://orcid.org/0000-0001-9109-3377)
- Nan Zhou (ORCID: https://orcid.org/0009-0005-4063-7764)
Institutions
- The University of Sydney (AU)
- Shanxi Medical University (CN)
- First Hospital of Shanxi Medical University (CN)
- National Center for Drug Screening (CN)
- China XD Group (China) (CN)
- Westmead Institute for Medical Research (AU)
- Xi'an Jiaotong University (CN)
Publication Details
- Journal
- Chinese Medicine
- Published
- 2025-08-19
- DOI
- https://doi.org/10.1186/s13020-025-01177-z
- Citations
- 1
- Primary Topic
- Piperaceae Chemical and Biological Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.56
Funders
- National Natural Science Foundation of China