Lock-in amplitude-phase correlations for enhanced imaging and segmentation in stimulated Raman scattering microscopy

Modulation transfer-based signal recovery methods, such as stimulated Raman scattering (SRS) microscopy, commonly use lock-in amplifier amplitude and phase outputs, with each providing readily accessible yet complementary information. Here we show that 2D amplitude-phase correlations enhance both imaging and segmentation (chemical specificity) in spectral focusing SRS microscopy, exemplified here by the characterization of an economic lithium ore sample. We additionally demonstrate that bilateral filtering applied to 2D amplitude-phase correlations leads to improved edge-preserving imaging.

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Publication Details

Journal
Optics Express
Published
2025-08-18
DOI
https://doi.org/10.1364/oe.569993
Primary Topic
Spectroscopy Techniques in Biomedical and Chemical Research
Type
article
Field-Weighted Citation Impact
0.00

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article

Lock-in amplitude-phase correlations for enhanced imaging and segmentation in stimulated Raman scattering microscopy

Alexander N. Harper, Albert Stolow, Adrian F. Pegoraro, Jonathan Boisvert et al.
Optics Express
Spectroscopy Techniques in Biomedical and Chemical Research
article

Lock-in amplitude-phase correlations for enhanced imaging and segmentation in stimulated Raman scattering microscopy

Alexander N. Harper, Albert Stolow, Adrian F. Pegoraro, Jonathan Boisvert, Tassos Grammatikopoulos
article en

Abstract

Modulation transfer-based signal recovery methods, such as stimulated Raman scattering (SRS) microscopy, commonly use lock-in amplifier amplitude and phase outputs, with each providing readily accessible yet complementary information. Here we show that 2D amplitude-phase correlations enhance both imaging and segmentation (chemical specificity) in spectral focusing SRS microscopy, exemplified here by the characterization of an economic lithium ore sample. We additionally demonstrate that bilateral filtering applied to 2D amplitude-phase correlations leads to improved edge-preserving imaging.

Optics ExpressVol. 33(19)
SGS (Canada) (CA), National Research Council Canada (CA)
University of Ottawa, Canada Research Chairs, Natural Sciences and Engineering Research Council of Canada, National Research Council Canada
Openalex Percentile: Top 85%
Spectroscopy Techniques in Biomedical and Chemical Research
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Lock-in amplitude-phase correlations for enhanced imaging and segmentation in stimulated Raman scattering microscopy — Alexander N. Harper, Albert Stolow, et al. · Optics Express (2025) | TGRS Research Map | TGRS