Species diversity estimation in a typical tropical forest: which phenological stage and spatial resolution are suitable?

Satellite remote sensing data is essential for large-scale, timely, and repeatable monitoring of forest species diversity. While various methods have been applied to satellite-based diversity estimation at regional scales, selecting suitable sensor and monitoring period remains challenging, especially in tropical forests. This study aims to identify the optimal time window, spatial resolution, and metrics for species diversity estimation in the Jianfengling tropical forest in southern China. We constructed stepwise linear regression models for estimating Richness, Simpson, and Shannon-Wiener indices using <i>in-situ</i> species diversity and heterogeneity metrics of spectra and structure. For analyzing phenology influence, we utilized six Sentinel-2 images acquired bimonthly from January to November. For evaluating scale dependency, we resampled the GF2 image to five spatial resolutions ranging from 0.8 to 10 m. The results indicated that the suitable phenological periods for species diversity estimation were at the beginning and end of the growing season, especially September performing the best for all diversity indices. Among four types of heterogeneity metrics, spectral information consistently explained most variance in species diversity indices across all periods. The optimal spatial resolution for estimating Richness and Shannon-Wiener index was 4-5 m, which corresponded to the average tree crown size. The texture features made a significant contribution compared to other metrics. Our study highlights that species diversity monitoring is highly dependent on the spatiotemporal scales of remote sensing data. It may offer practical guidance for selecting appropriate data and methods for species diversity monitoring in tropical forests.