Figure 4 from PSA Secretion from Single Circulating Tumor Cells of Metastatic Castration-Naïve Prostate Cancer Patients
<p>Single-cell seeding of CTCs in a nanowell chip. Images of nanowells after seeding of sorted DLA samples. Nanowells 1 and 2 show calcein<sup>+</sup> PSMA<sup>+</sup> CD45<sup>−</sup> cells and nanowells 3 and 4 show calcein<sup>+</sup> PSMA<sup>−</sup> CD45<sup>−</sup> cells. The green bar provides an estimate of the intensity levels per image. The cells that are stained brightly are viewed as positive. The red dashed line is set at the edge of the green bar of thumbnails 3 and 4. PSMA intensity (green bar) beyond this threshold indicates a positive signal as seen in the nanowells 1 and 2. CD45 intensity beyond this threshold indicates a positive signal and the presence of leukocytes in nanowells 5 and 6.</p>
Authors
- Leon W.M.M. Terstappen (ORCID: https://orcid.org/0000-0001-5944-3787)
- Danny Houtsma (ORCID: https://orcid.org/0000-0001-8792-0368)
- Brigitte C.M. Haberkorn (ORCID: https://orcid.org/0009-0006-6869-7264)
- Michiel Stevens (ORCID: https://orcid.org/0000-0001-8643-7235)
- Wendy M. van der Deure
- Nick Beije (ORCID: https://orcid.org/0000-0001-5462-9486)
- Paul Hamberg (ORCID: https://orcid.org/0000-0002-8223-0250)
- Khrystany T. Isebia (ORCID: https://orcid.org/0000-0002-3006-5206)
- Richell Booijink (ORCID: https://orcid.org/0000-0002-2270-1284)
- Eshwari Dathathri (ORCID: https://orcid.org/0000-0001-9389-029X)
- Peter A.W. te Boekhorst (ORCID: https://orcid.org/0000-0002-3566-5016)
- John W.M. Martens (ORCID: https://orcid.org/0000-0002-3428-3366)
- Martijn P. Lolkema (ORCID: https://orcid.org/0000-0003-0466-2928)
- Fikri Abali
- Jaco Kraan (ORCID: https://orcid.org/0000-0001-7445-7984)
- Ruchi Bansal (ORCID: https://orcid.org/0000-0003-2470-5876)
- Joan van den Bosch
- Tanja C. van Dijk
Publication Details
- Published
- 2025-08-18
- DOI
- https://doi.org/10.1158/2767-9764.29929879
- Primary Topic
- Prostate Cancer Treatment and Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00