Figure S3 from PSA Secretion from Single Circulating Tumor Cells of Metastatic Castration-Naïve Prostate Cancer Patients

<p>Visualization of the nanowells and membrane using the VyCAP SPOT software (A) Imprint of IgG antibody printed onto a PVDF membrane using a nanowell array, is used to align and match the secreted proteins to the corresponding cell inside a well. (B) The PSA proteins secreted by the individual CTC is printed onto PVDF membrane. (C) A brightfield image of the nanowell arrays filled with CTC. (D)Viable CTC inside individual wells of the chip. (E) PSMA-PE expression of the CTC inside the nanowells. (F) CD45-APC expression of the CTC inside the chip. Images in the lower left corner illustrate a small part of the membrane showing the IgG spots (A) and PSA spots (B) with their corresponding well-id analyzed in the VyCAP SPOT software. The corresponding CTC secreting PSA can now be located inside the nanowell array (C-F) to determine cell phenotype and isolated for downstream analysis.</p>

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Publication Details

Published
2025-08-18
DOI
https://doi.org/10.1158/2767-9764.29929861
Primary Topic
Prostate Cancer Treatment and Research
Type
article
Field-Weighted Citation Impact
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article

Figure S3 from PSA Secretion from Single Circulating Tumor Cells of Metastatic Castration-Naïve Prostate Cancer Patients

Leon W.M.M. Terstappen, Danny Houtsma, Brigitte C.M. Haberkorn, Michiel Stevens et al.
Prostate Cancer Treatment and Research
article

Figure S3 from PSA Secretion from Single Circulating Tumor Cells of Metastatic Castration-Naïve Prostate Cancer Patients

Leon W.M.M. Terstappen, Danny Houtsma, Brigitte C.M. Haberkorn, Michiel Stevens, Wendy M. van der Deure, Nick Beije, Paul Hamberg, Khrystany T. Isebia, Richell Booijink, Eshwari Dathathri, Peter A.W. te Boekhorst, John W.M. Martens, Martijn P. Lolkema, Fikri Abali, Jaco Kraan, Ruchi Bansal, Joan van den Bosch, Tanja C. van Dijk
article en

Abstract

<p>Visualization of the nanowells and membrane using the VyCAP SPOT software (A) Imprint of IgG antibody printed onto a PVDF membrane using a nanowell array, is used to align and match the secreted proteins to the corresponding cell inside a well. (B) The PSA proteins secreted by the individual CTC is printed onto PVDF membrane. (C) A brightfield image of the nanowell arrays filled with CTC. (D)Viable CTC inside individual wells of the chip. (E) PSMA-PE expression of the CTC inside the nanowells. (F) CD45-APC expression of the CTC inside the chip. Images in the lower left corner illustrate a small part of the membrane showing the IgG spots (A) and PSA spots (B) with their corresponding well-id analyzed in the VyCAP SPOT software. The corresponding CTC secreting PSA can now be located inside the nanowell array (C-F) to determine cell phenotype and isolated for downstream analysis.</p>

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Figure S3 from PSA Secretion from Single Circulating Tumor Cells of Metastatic Castration-Naïve Prostate Cancer Patients — Leon W.M.M. Terstappen, Danny Houtsma, et al. · (2025) | TGRS Research Map | TGRS