Dual-Phase Antidiabetic Efficacy of Cultivated Cordyceps sinensis: Mechanistic In-Vitro Validation and Therapeutic In-Vivo Outcomes

Cultivation is an effective conservation approach that can balance market demand and species protection. Wild and cultivated Cordyceps sinensis were investigated to understand possible variation in potential anti-diabetic-related bioactivity. Diabetes was induced in Wistar rats by the administration of a HFD for 15 days/STZ (35 mg/kg b.w., i.p.). Cordyceps sinen-sis at dose of 50 and 100 mg/kg b.w., p.o. was administered to diabetic rats for 28 days in HFD/STZ-induced Type 2 diabetic rats The effect of carbohydrate molecule of cultivated C. sinensis (CS) on blood glucose and body weight was studied in Type 2 diabetic rats. All these effects were compared with glibencl-amide (5 mg/kg b.w., p.o.) as a reference antidiabetic drug. HPLC and HPTLC analysis of water extracts of wild and laboratory adopted C. sinensis revealed similar properties and there was not any difference observed. The concentration of carbohydrate molecule in cultivated Cordyceps sinensis was 5% and the purity of extracted carbohydrate molecule from cultivated Cordyceps sinensis powder by HPLC was observed very high carbohydrate molecule of Cordyceps sinensis shows significant In-vitro antidiabetic activity. The administration of CS (50 and 100 mg/kg b.w., p.o.) resulted in a significant decrease in blood glucose level, body weight and significant increase in serum insulin level when compared to diabetic control. The results suggest that Cordyceps sinensis possesses a promising effect on the HFD/STZ -induced Type 2 diabetes.