In Vitro Assessment of the Effects of Endodontic Sealers on Cell Viability, Adhesion, and Migration
This study aimed to evaluate the in vitro cytocompatibility and bioactivity of the newly launched calcium silicate-based sealer BioRoot Flow, compared with other calcium silicate-based sealers (AH Plus Bioceramic Sealer and BioRoot RCS) and the epoxy resin-based AH Plus Jet. NIH/3 T3 fibroblasts were used to assess cell viability, adhesion, and migration. Material characterisation was performed using SEM, EDS, and XRD. BioRoot RCS exhibited the highest cell viability, while BioRoot Flow showed fewer adherent cells and reduced migration at 72 h. EDS revealed zirconium content in BioRoot Flow, and XRD confirmed calcite presence in all calcium silicate-based sealers. Variations in cell behaviour may be related to differences in chemical composition and surface properties, highlighting the need for further investigation.
Authors
- Ana Cristina Padilha Janini (ORCID: https://orcid.org/0000-0003-3058-8557)
- Anna Carolina Neves Leutz (ORCID: https://orcid.org/0009-0003-3006-6406)
- Victor Augusto Benedicto dos Santos (ORCID: https://orcid.org/0000-0003-1715-7146)
- Adriana de Jesus Soares (ORCID: https://orcid.org/0000-0002-8078-1606)
- Marina Angélica Marciano (ORCID: https://orcid.org/0000-0001-6244-2531)
- Brenda Paula Figueiredo de Almeida Gomes (ORCID: https://orcid.org/0000-0002-8449-0646)
- Talita Tartari (ORCID: https://orcid.org/0000-0003-4372-0158)
- Amanda Nowicki de Salles
Institutions
- Universidade Estadual de Campinas (UNICAMP) (BR)
- Methodist University of Piracicaba (BR)
Publication Details
- Journal
- Australian Endodontic Journal
- Published
- 2025-08-18
- DOI
- https://doi.org/10.1111/aej.70013
- Citations
- 1
- Primary Topic
- Endodontics and Root Canal Treatments
- Type
- article
- Field-Weighted Citation Impact
- 1.79
Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior