Analysis of the Bacterial Community and Fatty Acid Composition in the Bacteriome of the Lac Insect Llaveia axin axin

Microbial symbioses play crucial roles in insect physiology, contributing to nutrition, detoxification, and metabolic adaptations. However, the microbial communities associated with the lac insect <i>Llaveia axin axin</i>, an economically significant species used in traditional lacquer production, remain poorly characterized. In this study, the bacterial diversity and community structure of <i>L. axin axin</i> were investigated using both culture-dependent and culture-independent (metagenomic) approaches, combined with fatty acid profile analysis. The insects were bred at the laboratory level, in controlled conditions, encompassing stages from eggs to adult females. Bacterial strains were isolated from bacteriomes and identified through 16S rRNA gene amplification and genomic fingerprinting through ARDRA analysis. Metagenomic DNA was sequenced using the Illumina MiSeq platform, and fatty acid profiles were determined by gas chromatography-mass spectrometry (GC-MS). A total of 20 bacterial strains were isolated, with <i>Acinetobacter</i>, <i>Moraxella</i>, <i>Pseudomonas</i>, and <i>Staphylococcus</i> detected in first-instar nymphs; <i>Methylobacterium</i>, <i>Microbacterium</i>, and <i>Bacillus</i> in pre-adult females; and <i>Bacillus</i> and <i>Microbacterium</i> in adults. Metagenomic analysis revealed key genera including <i>Sodalis</i>, <i>Blattabacterium</i>, and <i>Candidatus Walczuchella</i>, with <i>Sodalis</i> being predominant in early stages and Blattabacteriaceae in adults. Fatty acid analysis identified palmitic, oleic, linoleic, arachidic, and stearic acids, with stearic acid being the most abundant. These results suggest that dominant bacteria contribute to lipid biosynthesis and metabolic development in <i>L. axin axin</i>.