Validation of human sensory neurons derived from inducible pluripotent stem cells as a model for latent infection and reactivation by herpes simplex virus 1

Herpes simplex viruses are prevalent human pathogens that cause significant morbidity and mortality in neonates and adults. They undergo acute infection in the mucosae and spread to peripheral neurons including sensory and autonomic neurons, where they establish lifelong latent infection. Reactivation from latency leads to recurrent infection and disease, including central nervous system infection that can be life-threatening. Experimental studies of HSV latency have mostly been conducted in animals, which may differ from the human situation. In this study, we establish a system for differentiation of human-inducible pluripotent stem cells into sensory neurons and for latent infection and reactivation by herpes simplex virus 1. This system will enable studies of the mechanism of HSV latent infection in human sensory neurons and therapeutic approaches to curtail it.

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Publication Details

Journal
mBio
Published
2025-08-18
DOI
https://doi.org/10.1128/mbio.01871-25
Citations
6
Primary Topic
Herpesvirus Infections and Treatments
Type
article
Field-Weighted Citation Impact
8.13

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article

Validation of human sensory neurons derived from inducible pluripotent stem cells as a model for latent infection and reactivation by herpes simplex virus 1

Elizabeth D. Buttermore, Clifford J. Woolf, Shu-Fan Chou, Jean M. Pesola et al.
6 citations
mBio
Herpesvirus Infections and Treatments
8.13
article

Validation of human sensory neurons derived from inducible pluripotent stem cells as a model for latent infection and reactivation by herpes simplex virus 1

Elizabeth D. Buttermore, Clifford J. Woolf, Shu-Fan Chou, Jean M. Pesola, Biswajit Das, Donald M. Coen, Alex H. M. Ng, David M. Knipe, Nicolás Romero, Hyung Suk Oh, Jennifer S. Lee, Jaehoon Shim, Priya Raja, George M. Church
article en
6 citations

Abstract

Herpes simplex viruses are prevalent human pathogens that cause significant morbidity and mortality in neonates and adults. They undergo acute infection in the mucosae and spread to peripheral neurons including sensory and autonomic neurons, where they establish lifelong latent infection. Reactivation from latency leads to recurrent infection and disease, including central nervous system infection that can be life-threatening. Experimental studies of HSV latency have mostly been conducted in animals, which may differ from the human situation. In this study, we establish a system for differentiation of human-inducible pluripotent stem cells into sensory neurons and for latent infection and reactivation by herpes simplex virus 1. This system will enable studies of the mechanism of HSV latent infection in human sensory neurons and therapeutic approaches to curtail it.

mBioVol. 16(9)
Boston Children's Hospital (US), Harvard University (US), Boston VA Research Institute (US), Inspire (CH)
Harvard Medical School, Boston Children's Hospital, National Institutes of Health
Good health and well-being
Openalex Percentile: Top 2%
Herpesvirus Infections and Treatments
8.13
DOIOpen Access PDFOpenAlex
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