GP2a I118 and GP4 D43 play critical roles in the attachment of PRRSV to the CD163 receptor: implications for anti-PRRSV infection targets
Currently, most modified live vaccines (MLVs) against animal diseases are derived from serial passages of parental virulent viruses in heterologous animal cells. This process enhances viral adaptation to heterologous cells while significantly reducing viral infectivity to host animal cells, thereby attenuating virulence in hosts. However, the mechanisms underlying the changes in tropism of many MLVs remain largely unknown. In this study, we identified and confirmed two key residues associated with changes in tropism. Importantly, we demonstrated that small peptides can block viral binding to receptors. These findings not only provide potential targets for the development of antiviral drugs or neutralizing antibodies but also offer valuable references for studying tropism changes in other viruses.
Authors
- Tongqing An (ORCID: https://orcid.org/0000-0003-4619-4587)
- Xuehui Cai (ORCID: https://orcid.org/0000-0001-9098-7782)
- Hao Song (ORCID: https://orcid.org/0000-0002-2811-0370)
- Xianghe Huang (ORCID: https://orcid.org/0009-0005-9227-0642)
- Haiwei Wang (ORCID: https://orcid.org/0000-0002-0588-2511)
- Meng Chen (ORCID: https://orcid.org/0009-0004-9442-8047)
- Shujie Wang (ORCID: https://orcid.org/0000-0002-7020-5410)
- Guoqing Liu (ORCID: https://orcid.org/0000-0003-0142-5978)
- Haojie Wang (ORCID: https://orcid.org/0000-0002-9552-6093)
- Yongbo Yang (ORCID: https://orcid.org/0000-0003-0907-8747)
- Xiaoxiao Tian
Institutions
- Heilongjiang University of Science and Technology (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Harbin Veterinary Research Institute (CN)
Publication Details
- Journal
- Journal of Virology
- Published
- 2025-08-18
- DOI
- https://doi.org/10.1128/jvi.00963-25
- Citations
- 4
- Primary Topic
- Animal Virus Infections Studies
- Type
- article
- Field-Weighted Citation Impact
- 4.77
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Heilongjiang Province
- National Key Research and Development Program of China
- Agricultural Science and Technology Innovation Program