Chemical characterization and antimicrobial potential of purified aceh patchouli oil (Pogostemon cablin Benth.) residue for floor cleaning product innovation

• Patchouli oil residue was characterized using GC-MS and found rich in patchouli alcohol. • Iron content in the residue was reduced by 60.7% using activated charcoal. • The residue showed antibacterial and antifungal activity against S. aureus, E. coli , and C. albicans . • A floor cleaning soap was formulated and met Indonesian National Standard (SNI 1842:2019). • The formulated product retained strong antimicrobial activity against tested microbes. Patchouli oil residue, a by-product of vacuum distillation, remains underutilized despite its bioactive compound content. This study aimed to characterize the residue using Gas Chromatography–Mass Spectrometry (GC-MS), purify iron (Fe) contamination using activated carbon, evaluate its antimicrobial and antifungal activities, and formulate it into a floor-cleaning soap. GC-MS analysis identified patchouli alcohol (57.19%) as the dominant compound. Purification using 5 g of activated carbon for 3 hours reduced Fe content from 10.351 mg/L to 4.062 mg/L (60.7% reduction), improved residue clarity, and eliminated naphthalene, a potential contaminant. The purified residue exhibited strong antibacterial activity against Staphylococcus aureus (13.28 mm ± 1.38) and moderate activity against Escherichia coli (7.02 mm ± 0.23), as well as strong antifungal activity against Candida albicans (14.88 mm ± 2.88). The formulated floor-cleaning soap met Indonesian National Standard (SNI 1842:2019) for pH, viscosity, specific gravity, foam stability, and homogeneity. It retained bioactivity with inhibition zones of 15.89 mm ( S. aureus ), 6.00 mm ( E. coli ), and 10.89 mm ( C. albicans ), showing enhanced antibacterial activity against S. aureus compared to the purified residue, but slightly lower activity against E. coli and C. albicans . These findings confirm that the formulated product not only meets national standards but also offers antimicrobial functionality. This study contributes a sustainable and scientific approach to valorizing agro-industrial waste into a value-added floor-cleaning product with confirmed antimicrobial and antifungal efficacy, aligning with green chemistry and circular economy principles.

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Publication Details

Journal
South African Journal of Chemical Engineering
Published
2025-08-18
DOI
https://doi.org/10.1016/j.sajce.2025.08.010
Primary Topic
Essential Oils and Antimicrobial Activity
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article
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article

Chemical characterization and antimicrobial potential of purified aceh patchouli oil (Pogostemon cablin Benth.) residue for floor cleaning product innovation

Binawati Ginting, Syaifullah Muhammad, Elly Sufriadi, Nadia Isnaini et al.
South African Journal of Chemical Engineering
Essential Oils and Antimicrobial Activity
article

Chemical characterization and antimicrobial potential of purified aceh patchouli oil (Pogostemon cablin Benth.) residue for floor cleaning product innovation

Binawati Ginting, Syaifullah Muhammad, Elly Sufriadi, Nadia Isnaini, Vicky Prajaputra, Ernawati Ernawati, Hanifa Rifdah Aiman
article en

Abstract

• Patchouli oil residue was characterized using GC-MS and found rich in patchouli alcohol. • Iron content in the residue was reduced by 60.7% using activated charcoal. • The residue showed antibacterial and antifungal activity against S. aureus, E. coli , and C. albicans . • A floor cleaning soap was formulated and met Indonesian National Standard (SNI 1842:2019). • The formulated product retained strong antimicrobial activity against tested microbes. Patchouli oil residue, a by-product of vacuum distillation, remains underutilized despite its bioactive compound content. This study aimed to characterize the residue using Gas Chromatography–Mass Spectrometry (GC-MS), purify iron (Fe) contamination using activated carbon, evaluate its antimicrobial and antifungal activities, and formulate it into a floor-cleaning soap. GC-MS analysis identified patchouli alcohol (57.19%) as the dominant compound. Purification using 5 g of activated carbon for 3 hours reduced Fe content from 10.351 mg/L to 4.062 mg/L (60.7% reduction), improved residue clarity, and eliminated naphthalene, a potential contaminant. The purified residue exhibited strong antibacterial activity against Staphylococcus aureus (13.28 mm ± 1.38) and moderate activity against Escherichia coli (7.02 mm ± 0.23), as well as strong antifungal activity against Candida albicans (14.88 mm ± 2.88). The formulated floor-cleaning soap met Indonesian National Standard (SNI 1842:2019) for pH, viscosity, specific gravity, foam stability, and homogeneity. It retained bioactivity with inhibition zones of 15.89 mm ( S. aureus ), 6.00 mm ( E. coli ), and 10.89 mm ( C. albicans ), showing enhanced antibacterial activity against S. aureus compared to the purified residue, but slightly lower activity against E. coli and C. albicans . These findings confirm that the formulated product not only meets national standards but also offers antimicrobial functionality. This study contributes a sustainable and scientific approach to valorizing agro-industrial waste into a value-added floor-cleaning product with confirmed antimicrobial and antifungal efficacy, aligning with green chemistry and circular economy principles.

South African Journal of Chemical EngineeringVol. 54
Universitas Syiah Kuala (ID)
Industry, innovation and infrastructure
Openalex Percentile: Top 75%
Essential Oils and Antimicrobial Activity
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