In Vitro Germination of Nicotiana glauca: Unlocking Its Biotechnological Potential

Nicotiana glauca Graham (Solanaceae) produces anabasine with biotechnological applications. The propagation and utilization of N. glauca are hindered by seed dormancy and sensitivity to storage conditions. To address this challenge, this study investigated the effects of storage temperature and osmotic regulation with sorbitol on in vitro seed germination and plant establishment. Objectives: The aim was to optimize temperature storage protocols and in vitro culture conditions to improve germination rates and plant growth. Method: Seeds were stored at various temperatures (room temperature, 6°C, and -4°C) and germinated with different concentrations of sorbitol. Germination rates, plant height, and biomass were assessed. Results showed that low-temperature storage at 6°C, combined with sorbitol treatment, significantly enhanced seed viability and germination rates. This suggests that sorbitol can play a role in mitigating osmotic stress. In contrast, storage at -4°C led to decreased viability, highlighting the importance of temperature control. Conclusions: This study provides valuable insights into the factors affecting N. glauca storage seed and germination. The findings contribute to the development of efficient propagation protocols and the potential for biotechnological applications of this species.