Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR
The results obtained in this study are promising. Direct RT-qPCR for DENV detection in patient sera, could make PCR-based dengue detection and typing, and potentially other target detections, more affordable for reference laboratories in LMICs by reducing reagent cost by approximately two-thirds. Further studies are needed to evaluate DENV direct RT-qPCR on prospective samples in diagnosis context and to improve the sensitivity by minimizing the impact of inhibitors.
Authors
- Audrey Dubot‐Pérès (ORCID: https://orcid.org/0000-0003-1267-0725)
- Manivanh Vongsouvath (ORCID: https://orcid.org/0000-0002-2249-7367)
- Elizabeth A. Ashley (ORCID: https://orcid.org/0000-0002-7620-4822)
- Malavanh Vongsouvath
- Vilayouth Phimolsannousith
- Padthana Kiedsathid
Institutions
- Inserm (FR)
- Mahosot Hospital (LA)
- University of Oxford (GB)
- Délégation Régionale Provence-Alpes-Côte d’Azur et Corse (FR)
- Institut de Recherche pour le Développement (FR)
Publication Details
- Journal
- PLoS ONE
- Published
- 2025-08-18
- DOI
- https://doi.org/10.1371/journal.pone.0330459
- Primary Topic
- Mosquito-borne diseases and control
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Aix-Marseille Université
- Wellcome Trust