Autofluorescence Quenching in Decellularized Plant Scaffolds for Tissue Engineering

Copper sulfate is a highly effective and stable quenching agent for reducing autofluorescence in plant-derived scaffolds. While suitable for post-fixation imaging, scaffold-specific effects on viability limit its use in live-cell applications. Autofluorescence reduction was achieved without compromising scaffold mechanics. Ammonium chloride and sodium borohydride may be preferable when preserving cell viability is a priority.