In Vitro Study with Addition of Different Levels of Melissa on Rumen Fermentation Characteristics, Digestibility and Methane Production

This study aimed to evaluate the effect of adding Melissa aromatic plant at 0%, 8%, and 10% from the total dry matter (DM) In the middle of the nursery for the liquid rumen model in the laboratory . A mixture ration of 200 mg of concentrate and roughage (80:20) was added to a syringe bottle, where rumen fluid with artificial saliva were pipetted in and incubated at 39°C to assess organic matter and dry matter digestibility, total gas and methane production, and rumen fermentation characteristics. The results showed that there were no significant differences between the treatments of all levels of lemon balm when using different levels of concentrated feed to roughage, which may be due to the lack of effect of these levels of addition on the activity of microorganisms in digesting dry matter and organic matter and the Total gas production and methane gas in this study.