Pharmaco-toxicological assessment of the effect of the rachipred supplement on morphofunctional blood parameters in laboratory rats

The purpose of the research is to study the toxicological safety of the original protein-mineral supplement. The objectives of the research included studying the dynamics of morphofunctional blood parameters of laboratory rats in the framework of a study of chronic toxicity against the background of the use of the original protein-mineral supplement rachipred. The additive contained a protein component of natural origin, calcium and phosphorus compounds of natural origin, and crystalline sulfur. The experiment was carried out on 30 laboratory female White rats with an initial weight of 200-230 g. 3 groups were formed (n=10). The control group received a placebo, the 1st experimental group consumed the supplement in a therapeutic dosage of 1 g/kg of body weight, the 2nd experimental group received a five-fold dose from the therapeutic group. The duration of the experiment was 90 days. The use of a feed additive in a therapeutic dosage and a fivefold increase in the dose does not cause negative effects on hematological parameters. At the same time, in both experimental groups, in relation to the values of the control group animals within the reference boundaries, it was found that the total number of leukocytes increased by 54.1% in group 1 and 56.1% in group 2; an increase in the number of erythrocytes in the 1st and 2nd experimental groups – by 17.1% and 12.0%, respectively; an increase in hemoglobin concentration – by 4.5% for group 1 and 6.4% (P0.05) for group 2; an increase in hematocrit value – the difference in the 1st experimental group was 13.1% (P0.01), in 2nd experimental – 10.4% (P0.05); the platelet count increases – the values in the experimental groups were higher than in the control by 13.48% and 14.11% in the 1st and 2nd experimental groups, respectively.