Silica‐Assisted Solid‐Phase Peptide Synthesis (SiPPS)
Non-swelling silica-based resin was used for peptide synthesis. The strategy used is similar to that of solid-phase peptide synthesis (SPPS), referred to as silica-assisted solid-phase peptide synthesis (SiPPS). A 2-h coupling seemed to favor the coupling compared to that of 1-h standard coupling. The use of this non-swelling resin allows a 50% reduction in the consumption of solvents. The strategy was well demonstrated for the synthesis of H-YSSFL-NH<sub>2</sub>, linear oxytocin, angiotensin II, and afamelanotide using a silica-based support (Fmoc-Rink amide SiliaBond manufactured from SiliCycle Inc.). The peptides were found to have more than acceptable purity, although there was a loss in overall yields.
Authors
- Beatriz G. de la Torre (ORCID: https://orcid.org/0000-0001-8521-9172)
- Ntombifikile M. Nkwanyana (ORCID: https://orcid.org/0000-0001-5903-2400)
- Fernando Alberício (ORCID: https://orcid.org/0000-0002-8946-0462)
- Steeves Potvin
- Ashish Kumar (ORCID: https://orcid.org/0000-0001-5632-2293)
- Anamika Sharma (ORCID: https://orcid.org/0000-0003-1282-5838)
- François Béland (ORCID: https://orcid.org/0000-0002-2031-3317)
- Brunello Nardone
- Amit Chakraborty (ORCID: https://orcid.org/0000-0002-1244-304X)
- Georges Thibaut‐Koumba
Institutions
- SiliCycle (Canada) (CA)
- Universitat de Barcelona (ES)
- University of KwaZulu-Natal (ZA)
Publication Details
- Journal
- Journal of Peptide Science
- Published
- 2025-08-16
- DOI
- https://doi.org/10.1002/psc.70052
- Citations
- 2
- Primary Topic
- Chemical Synthesis and Analysis
- Type
- article
- Field-Weighted Citation Impact
- 1.61