Traction force and mechanosensitivity mediate species-specific implantation patterns in human and mouse embryos
The invasion of human embryos in the uterus overcoming the maternal tissue barrier is a crucial step in embryo implantation and subsequent development. Although tissue invasion is fundamentally a mechanical process, most studies have focused on the biochemical and genetic aspects of implantation. Here, we fill the gap by using a deformable ex vivo platform to visualize traction during human embryo implantation. We demonstrate that embryos apply forces remodeling the matrix with species-specific displacement amplitudes and distinct radial patterns: principal displacement directions for mouse embryos, expanding on the surface while human embryos insert in the matrix generating multiple traction foci. Implantation-impaired human embryos showed reduced displacement, as well as mouse embryos with inhibited integrin-mediated force transmission. External mechanical cues induced a mechanosensitive response, human embryos recruited myosin, and directed cell protrusions, while mouse embryos oriented their implantation or body axis toward the external cue. These findings underscore the role of mechanical forces in driving species-specific invasion patterns during embryo implantation.
Authors
- Marc Casals (ORCID: https://orcid.org/0000-0002-5174-8452)
- Julien Colombelli (ORCID: https://orcid.org/0000-0002-2784-4276)
- Maria Demestre (ORCID: https://orcid.org/0000-0002-3672-5703)
- Anna Ferrer‐Vaquer (ORCID: https://orcid.org/0000-0002-7089-5730)
- Ot Massafret (ORCID: https://orcid.org/0000-0002-7511-0568)
- Samuel Ojosnegros (ORCID: https://orcid.org/0000-0002-9562-3264)
- Oren Tchaicheeyan
- Amélie Luise Godeau (ORCID: https://orcid.org/0000-0002-8778-0120)
- Anna Seriola (ORCID: https://orcid.org/0000-0003-4593-5195)
- Anna Veiga (ORCID: https://orcid.org/0000-0002-0943-9904)
- Ayelet Lesman (ORCID: https://orcid.org/0000-0003-2511-4381)
- Jordi Comelles (ORCID: https://orcid.org/0000-0002-9297-830X)
- Montserrat Boada (ORCID: https://orcid.org/0000-0003-0389-0638)
- Elena Martínez (ORCID: https://orcid.org/0000-0002-6585-4213)
- Denitza Denkova (ORCID: https://orcid.org/0000-0001-9811-1646)
- Miquel Solé (ORCID: https://orcid.org/0000-0003-4476-2165)
- Shahar Goren (ORCID: https://orcid.org/0009-0009-2233-649X)
- Albert Parra (ORCID: https://orcid.org/0009-0008-7225-4583)
- Ester Aroca
Institutions
- Tel Aviv University (IL)
- Hospital Universitario Dexeus (ES)
- Centro de Investigación Biomédica en Red (ES)
- Institute for Research in Biomedicine (ES)
- USP Institut Universitari Dexeus (ES)
- Center of Regenerative Medicine in Barcelona (ES)
- Institute for Bioengineering of Catalonia (ES)
- Universitat de Barcelona (ES)
Publication Details
- Journal
- Science Advances
- Published
- 2025-08-15
- DOI
- https://doi.org/10.1126/sciadv.adr5199
- Citations
- 7
- Primary Topic
- Reproductive System and Pregnancy
- Type
- article
- Field-Weighted Citation Impact
- 5.58