UV-Visible Spectrophotometric Method Development and Validation for the Estimation of Aqueous Chromolaena odorata

Background: Chromolaena odorata (Siam weed) is a perennial shrub used in the treatment of many ailments and disease conditions, also known as Siam weed. A potential weed of tropical and sub-tropical regions inhabits waterways. The medicinal values of Chromolaena odorata lie in their phytochemical's component. The present study aims to develop and validate an UV spectrophotometric method according to ICH guidelines for the estimation of C. odorata. Materials and Method: The study is focused on generating a specific, linear, accurate and precise UV spectrophotometric method for estimation of aqueous C. odorata. The method's parameters were assessed within a concentration range of 0.2 µg/ml - 1 µg/ml, with recovery rates specifying accuracy and low % relative standard deviation (RSD) values confirming precision. Limits of detection (LOD) and quantification (LOQ) for C. odorata were determined. Results and Discussion: Validation is conducted to ensure that the analytical methods are accurate, specific, reproducible, and resistant to the analyte range to be analyzed. The method was validated for many parameters. Solvent development step involves the use of water in which Siam showed spectrum with maximum absorbance at 211nm. A coefficient of correlation of 0.9975 was discovered in accordance with Beer's law. After determining the technique's sensitivity, the limit of detection and limit of quantification were determined to be 1.07 µg/ml and 3.25 µg/ml, respectively. Conclusion: In conclusion, the development and validation of a UV-visible spectrophotometric method for the estimation of aqueous Chromolaena odorata extracts demonstrate a promising approach for routine quality control analysis. The method offers a simple, cost-effective, and efficient means of analysis when adequately optimized. The validation according to ICH Q2 (R1) guidelines confirms that the method meets the necessary acceptance criteria, making it suitable for the routine analysis of Chromolaena odorata.