An immune-focused supplemental alignment pipeline captures information missed from dominant single-cell RNA-seq analyses, including allele-specific MHC-I regulation

Combining nimble data with standard pipelines enhances the fidelity and accuracy of experiments, maximizing the value of expensive datasets, and identifying cellular subsets not possible with standard tools alone.

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Publication Details

Journal
Frontiers in Immunology
Published
2025-08-08
DOI
https://doi.org/10.3389/fimmu.2025.1596760
Primary Topic
Single-cell and spatial transcriptomics
Type
article
Field-Weighted Citation Impact
0.00

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article

An immune-focused supplemental alignment pipeline captures information missed from dominant single-cell RNA-seq analyses, including allele-specific MHC-I regulation

William Goodwin, Carla Boyle, Daniel N. Streblow, Caralyn S. Labriola et al.
Frontiers in Immunology
Single-cell and spatial transcriptomics
article

An immune-focused supplemental alignment pipeline captures information missed from dominant single-cell RNA-seq analyses, including allele-specific MHC-I regulation

William Goodwin, Carla Boyle, Daniel N. Streblow, Caralyn S. Labriola, Afam A. Okoye, Aaron Barber-Axthelm, Jonah B. Sacha, Michael K. Axthelm, S.A. Benjamin, Nicole N. Haese, Derick M. Duell, GW McElfresh, Scott G. Hansen, Tyler James Bennett, Courtney Waytashek, Candice Nkoy, Andrea N. Selseth, Helen L. Wu, Benjamin N. Bimber, R. Keith Reeves, Gregory J. Boggy, Louis J. Picker, Jeremy Smedley, Sohita Ojha, Benjamin D. Varco-Merth, Shana Feltham, Maanasa Kaza
article en

Abstract

Combining nimble data with standard pipelines enhances the fidelity and accuracy of experiments, maximizing the value of expensive datasets, and identifying cellular subsets not possible with standard tools alone.

Frontiers in ImmunologyVol. 16
Oregon National Primate Research Center (US), Oregon Health & Science University (US), Duke University (US), Children's Cancer Therapy Development Institute (US)
Bill and Melinda Gates Foundation, National Institutes of Health, Oregon National Primate Research Center, NIH Office of the Director, National Institute of Allergy and Infectious Diseases
Good health and well-being
Openalex Percentile: Top 81%
Single-cell and spatial transcriptomics
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