DMP1-mediated transformation of DPSCs to CD31+/CD144+ cells demonstrate endothelial phenotype both in vitro and in vivo
The findings of this study indicate that the CD31<sup>+</sup>/CD144<sup>+</sup> fraction, retains both the phenotypic and functional characteristics of ECs, in contrast to the CD31<sup>-</sup>/CD144<sup>-</sup> fraction. Furthermore, <i>in vivo</i> analysis of the sorted ECs using the subcutaneous implantation model exhibited neovascularization along with the expression of vasculogenic markers. Overall, DPSC-derived ECs obtained by stimulation with DMP1 and cultured on HUVEC-ECM function as typical vascular ECs. This strategy, could be exploited for the development of vasculogeneis and as a therapeutic potential for tissue repair and regeneration.
Authors
- Velavan Bakthavachalam
- Amudha Ganapathy (ORCID: https://orcid.org/0000-0002-4484-9204)
- Yinghua Chen (ORCID: https://orcid.org/0000-0002-5020-7507)
- Anne George (ORCID: https://orcid.org/0000-0002-8486-8186)
Institutions
- University of Illinois Chicago (US)
Publication Details
- Journal
- Frontiers in Cell and Developmental Biology
- Published
- 2025-08-08
- DOI
- https://doi.org/10.3389/fcell.2025.1630129
- Citations
- 1
- Primary Topic
- Mesenchymal stem cell research
- Type
- article
- Field-Weighted Citation Impact
- 1.63