Antimicrobial Activity of Lacticaseibacillus rhamnosus CRL 2244 Extracts Against Community- and Hospital-Acquired Staphylococcus aureus

<b>Background/Objectives:</b> Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) remains a critical public health concern due to its multidrug resistance and capacity to form persistent infections, particularly in the context of implanted medical devices. Alternative therapeutic strategies that target bacterial virulence instead of viability are increasingly explored. This study aimed to evaluate the antimicrobial and antivirulence activity of an extract derived from <i>Lacticaseibacillus rhamnosus</i> CRL 2244 against two MRSA strains-USA300 and M86-and to elucidate its effects on bacterial physiology and gene expression under host-mimicking conditions. <b>Methods</b>: Antimicrobial activity was assessed using agar diffusion, MIC, and time-kill assays. Scanning electron microscopy of cells exposed to the extract confirmed decreased cellular density and morphological changes. Phenotypic assays evaluated biofilm formation, staphyloxanthin production, and adhesion to fibronectin. RT-qPCR analyzed transcriptional responses. Viability was assessed in the presence of human serum and type I collagen. <b>Results</b>: The CRL 2244 extract demonstrated bactericidal activity with up to 6-log₁₀ CFU/mL reduction at 1× MIC. In USA300, the extract reduced the expression of <i>hla</i>, <i>lukAB</i>, <i>fnbA</i>, and <i>icaA</i>, correlating with decreased staphyloxanthin levels. In M86, a significant reduction in biofilm formation and repression of <i>lukAB</i>, <i>nucA</i>, and <i>fnbA</i> were observed. Adhesion to fibronectin was impaired in both strains. The extract showed no cytotoxicity in human serum but reduced viability in collagen-enriched conditions. <b>Conclusions</b>: The <i>Lcb. rhamnosus</i> CRL 2244 extract modulates MRSA virulence in a strain-specific manner, targeting key regulatory and structural genes without inducing cytotoxic effects.