Spleen Transcriptome profiling shows varying immune reactions to poly (I:C) and LPS challenge in the Ussuri catfish (Pseudobagrus ussuriensis)

Poly(I:C) and lipopolysaccharide (LPS), two well-known pathogen-associated molecular patterns of viruses and bacteria, respectively, are frequently utilized to study host immunological responses to infections. Analysis of the gene expression profile in the spleen of Ussuri catfish ( Pseudobagrus ussuriensis ) infected with poly(I:C) or LPS at 0, 3, 12, and 24 h was the goal of the current study. After infection with LPS, each comparison group shared 34 differentially expressed genes (DEGs), but after infection with poly(I:C), 816 DEGs were shared. Following LPS or poly(I:C) infection, 10 DEGs were expressed differently in each group. The ECM-receptor interaction route and the cytokine-cytokine receptor interaction pathway were found to be the most enriched pathways for DEGs across all groups. B-type lectin , Protein FAM110B , CDK17 , Lumican , Netrin-4 , and nuclear receptor subfamily 4 group A member 2 ( NR4A2 ) all showed significant variations in their expression levels. Potential expression patterns found in the study might be helpful diagnostic tools for monitoring Ussuri catfish illness. • Short-term immune stimulation alters spleen genes expression. • 10 shared DEGs were differentially expressed in all compared groups. • The ECM-receptor interaction pathway and cytokine-cytokine receptor interaction pathway were the most enrichment pathway. • Potential biomarker candidates for monitoring Ussuri catfish diseases.