Comparison of Enzyme-Linked Immunosorbent Assay (ELISA) and Colloidal Gold Immunochromatographic Assay (GICA) for Tetracycline Detection

The rising use of tetracycline in China is leading to increased environmental pollution, highlighting the need for rapid, simple, and sensitive detection methods for monitoring. This study addresses the urgent requirement to track tetracycline contamination, which threatens ecological systems and human health. It specifically compares the effectiveness of two rapid detection methods: Enzyme-Linked Immunosorbent Assay (ELISA) and Colloidal Gold Immunochromatographic Assay (GICA), focusing on sensitivity, specificity, and field practicality. The methodology involves systematically comparing ELISA and GICA by assessing detection limits, linear ranges, and operational complexities through environmental water samples. Statistical analyses will evaluate the reliability and accuracy of each method, offering insights into their applicability for monitoring tetracycline pollution. This research aims to enhance strategies for environmental monitoring and pollution control concerning antibiotic residues. ELISA and GICA are rapid detection methods based on antigen-antibody binding; ELISA provides high sensitivity and specificity but involves complex procedures, while GICA offers ease of interpretation, reagent stability, and portability, albeit with a broader linear range and lower detection limit.