Comparison of pathogen detection performance between metagenomic next-generation sequencing and conventional culture in organ preservation fluids and recipient wound drainage fluids
By employing mNGS to analyze drainage fluids and organ preservation fluids, highly pathogenic and atypical pathogenic microorganisms can be rapidly identified with high throughput. While limitations exist in detecting fungi and Gram-positive bacteria, mNGS are need to be jointly applied with conventional culture under current conditions.
Authors
- Chen Gao (ORCID: https://orcid.org/0000-0003-3595-9839)
- Wenjia Yuan
- Jiyuan Li (ORCID: https://orcid.org/0000-0002-4766-677X)
- Wei Cao (ORCID: https://orcid.org/0000-0001-6133-1020)
- Gongbin Lan (ORCID: https://orcid.org/0000-0001-6500-5820)
- Longkai Peng (ORCID: https://orcid.org/0000-0002-3189-6511)
- Lei Liu (ORCID: https://orcid.org/0009-0008-5132-7093)
- Yu Wang (ORCID: https://orcid.org/0000-0001-9070-6004)
- Xuejing Zhu (ORCID: https://orcid.org/0000-0003-4101-9763)
- Yang Han (ORCID: https://orcid.org/0000-0002-8843-6970)
- Lei Song
- Xubiao Xie
- Shaojie Yu (ORCID: https://orcid.org/0000-0003-2202-3935)
- Liang Tan (ORCID: https://orcid.org/0000-0002-7481-5670)
- Ruobing Liang (ORCID: https://orcid.org/0009-0007-7683-2240)
- H. M. Dai (ORCID: https://orcid.org/0000-0002-1582-4593)
- Fenghua Peng (ORCID: https://orcid.org/0009-0001-1465-1950)
Institutions
- Central South University (CN)
- Second Xiangya Hospital of Central South University (CN)
- Atotech (United States) (US)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2025-08-11
- DOI
- https://doi.org/10.3389/fcimb.2025.1563962
- Primary Topic
- Renal Transplantation Outcomes and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- China Primary Health Care Foundation