A light-controlled one-tube detection platform combining CRISPR-Cas12a and RPA: an innovative approach for rapid diagnosis of Acinetobacter baumannii
We have successfully developed a light-controlled one-tube RPA-CRISPR/Cas12a detection system. It simplifies the operation and at the same time greatly reduces the risk of laboratory contamination caused by repeated tube opening, providing a new idea for the development of point-of-care testing (POCT).
Authors
- Xuebin Li
- Guijiang Wei (ORCID: https://orcid.org/0009-0004-5473-009X)
- Shihua Luo (ORCID: https://orcid.org/0009-0008-5701-6771)
- Lina Liang
- Jiangmei Ma
- Baoyan Ren
- Lele Pan
- Zihan Zhou
Institutions
- Guangxi Minzu University (CN)
- ZTE (China) (CN)
- Affiliated Hospital of Youjiang Medical University for Nationalities (CN)
- Baise University (CN)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2025-08-11
- DOI
- https://doi.org/10.3389/fbioe.2025.1663915
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China