Aptamer-functionalized magnetic covalent organic frameworks for rapid extraction and LC-MS/MS quantification of gentamicin in milk
Gentamicin (GEN) residues in food products, arising from its extensive use in veterinary medicine, have prompted stringent regulations and a demand for simple, rapid detection methods. In this study, aptamer-modified magnetic covalent organic frameworks (COFs), denoted as TpDBD@Fe₃O₄@apt, were synthesized and employed as adsorbents in magnetic solid-phase extraction (MSPE) for the highly specific capture and enrichment of GEN residues in milk. Several factors influencing MSPE efficiency were systematically evaluated and optimized. Coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS), the developed method exhibited good linearity within the linear range (R<sup>2</sup> ≥ 0.999), low detection limits (0.48-0.54 ng/mL), satisfactory recoveries (92.7 %-108.8 %), and high precision (RSDs ≤10.8 %). The proposed method was successfully applied to real milk samples, providing an efficient solution for the detection of GEN residues.
Authors
- Yangjiayi Xiang (ORCID: https://orcid.org/0000-0003-4584-666X)
- Cong Hu
- Hao Cao (ORCID: https://orcid.org/0000-0002-5204-5054)
- Gou Wu
- Hongmei Liu (ORCID: https://orcid.org/0000-0003-4620-8766)
- Yan Li (ORCID: https://orcid.org/0000-0002-3693-6121)
Institutions
- Jinshan Hospital of Fudan University (CN)
- Humanwell Healthcare Group (China) (CN)
- Pudong Medical Center (CN)
- Obstetrics and Gynecology Hospital of Fudan University (CN)
Publication Details
- Journal
- Food Chemistry
- Published
- 2025-08-10
- DOI
- https://doi.org/10.1016/j.foodchem.2025.145889
- Citations
- 1
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Natural Science Foundation of China
- Science and Technology Commission of Shanghai Municipality