Karyotype and fluorescent banding in the interspecific F1 hybrid Passiflora 'Lamepiana', confirmed by GISH

Abstract Passiflora 'Lamepiana' is the F1 hybrid from the cross between P. vitifolia and P. hatschbachii with ornamental characteristics. Based on cytogenetics techniques, this study aimed to confirm interspecific hybridization and characterize the karyotype of the hybrids and their parents using cytogenetic techniques. For all genotypes the predominance of metacentric chromosomes with two satellite pairs and a diploids karyotypes (2n = 18) were observed. It was possible to determine the occurrence of two satellite pairs in both the parents of the hybrids. The use of simultaneous probes of the F1 parents prepared according to GISH technique allowed to identify two chromosome lots each one inherited from both parents, confirming the hybrid character of the plants. Besides confirming hybridization, GISH did not demonstrate the existence of visible chromosomal alterations but rather cytogenetic stability in the hybrids.