Analysis of the DNA interaction activity of a Zn(II) phthalocyanine compound containing a peripheral tetra-4-(2-(N’-cyanoethyl)aminoethylsulfanyl) group

Tetra 4-(2-(N’-cyanoethyl)aminoethylsulphanyl) substituted Zn(II) phthalocyanine compound had been earlier generated and characterised. Absorption titration in the UV/Visible, fluorescence replacement experiments using the ethidium bromide as an intercalator, gel electroporesis, thermal melting and viscosity measurements were utilised to assess DNA binding property of ZnPc against CT-DNA at a pH of 7.37. It was concluded that ZnPc phthalocyanine complex exhibits a significant affinity for CT-DNA through intercalation interaction, having a binding constant of 2.83 × 106 M− 1. In addition to absorption titration assay, DNA binding efficiency of ZnPc was also evaluated by means of fluorescence titration and data obtained support the finding of absorption finding. The thermal denaturation profile of DNA was found to alter when zinc phthalocyanine was added and findings verified that the complex interacts with DNA. The interacting activity of ZnPc on CT-DNA was examined in the presence and absence of the compound by electrophoresis and viscosity experiments. The methods have validated that the compound ZnPc has an interacting activity on the DNA molecule. All the assays used suggested that ZnPc bounds to CT-DNA by intercalating. All these parameters refer that ZnPc may have a potential for photodynamic treatment.