The Effect of Concentrated Seawater Salinity on Soybean Protein Coagulation in Tofu Production

Seawater, which has a salinity of 35 ‰, contains essential ions such as chloride, sodium, sulfate, magnesium, and calcium. These ions play a crucial role in the coagulation of proteins. Salt-based coagulants are among the oldest and most commonly used in tofu production. Additionally, several metal cations exhibit similar coagulating effects on soybean proteins. Tofu can be produced by adding salt coagulants, like calcium sulfate (commonly known as tofu stone) and seawater extract. The seawater extract was obtained from seawater through evaporation in three distinct ponds, with varying evaporation times that can lead to differences in salinity and density. In this experiment, we used coagulants from these three ponds, labeled A, B, and C. Coagulant C, derived from the pond with the longest evaporation time, has the highest salinity of 310 ‰ and a density of 1.220 g/cm³. The mass of the tofu produced shows a consistent pattern among coagulants A, B, and C: an initial increase followed by a decrease, which is influenced by the salting-out and salting-in processes. When used at a volume of 15 mL, Coagulant C yielded the highest mass at 179.426 grams and the lowest water content at 71.152%, demonstrating its effectiveness in protein coagulation.

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Publication Details

Journal
Indonesian Chimica Letters
Published
2025-06-23
DOI
https://doi.org/10.19184/icl.v4i1.5885
Primary Topic
Enzyme Production and Characterization
Type
article
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article

The Effect of Concentrated Seawater Salinity on Soybean Protein Coagulation in Tofu Production

Yeni Maulidah Muflihah, Wuryanti Handayani, Asnawati Asnawati, Tri Mulyono et al.
Indonesian Chimica Letters
Enzyme Production and Characterization
article

The Effect of Concentrated Seawater Salinity on Soybean Protein Coagulation in Tofu Production

Yeni Maulidah Muflihah, Wuryanti Handayani, Asnawati Asnawati, Tri Mulyono, Manis Rohmawati
article en

Abstract

Seawater, which has a salinity of 35 ‰, contains essential ions such as chloride, sodium, sulfate, magnesium, and calcium. These ions play a crucial role in the coagulation of proteins. Salt-based coagulants are among the oldest and most commonly used in tofu production. Additionally, several metal cations exhibit similar coagulating effects on soybean proteins. Tofu can be produced by adding salt coagulants, like calcium sulfate (commonly known as tofu stone) and seawater extract. The seawater extract was obtained from seawater through evaporation in three distinct ponds, with varying evaporation times that can lead to differences in salinity and density. In this experiment, we used coagulants from these three ponds, labeled A, B, and C. Coagulant C, derived from the pond with the longest evaporation time, has the highest salinity of 310 ‰ and a density of 1.220 g/cm³. The mass of the tofu produced shows a consistent pattern among coagulants A, B, and C: an initial increase followed by a decrease, which is influenced by the salting-out and salting-in processes. When used at a volume of 15 mL, Coagulant C yielded the highest mass at 179.426 grams and the lowest water content at 71.152%, demonstrating its effectiveness in protein coagulation.

Indonesian Chimica LettersVol. 4(1)
Life below water
Openalex Percentile: Top 77%
Enzyme Production and Characterization
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The Effect of Concentrated Seawater Salinity on Soybean Protein Coagulation in Tofu Production — Yeni Maulidah Muflihah, Wuryanti Handayani, et al. · Indonesian Chimica Letters (2025) | TGRS Research Map | TGRS