Isolation of Pyricularia and preparation of DNA for Illumina Sequencing v1

This protocol describes procedures for isolating pure Pyricularia cultures from lesions in just 24 h and then generating DNA of sufficient purity for Illumina library production. The typical yield of purified DNA from 10 ml of culture is 5-10 µg.

Authors

Institutions

Publication Details

Published
2025-06-30
DOI
https://doi.org/10.17504/protocols.io.14egnrb7pl5d/v1
Citations
1
Primary Topic
Plant Disease Resistance and Genetics
Type
article
Field-Weighted Citation Impact
1.75
Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Isolation of Pyricularia and preparation of DNA for Illumina Sequencing v1

Mark Farman
1 citations
Plant Disease Resistance and Genetics
1.75
article

Isolation of Pyricularia and preparation of DNA for Illumina Sequencing v1

Mark Farman
article en
1 citations

Abstract

This protocol describes procedures for isolating pure Pyricularia cultures from lesions in just 24 h and then generating DNA of sufficient purity for Illumina library production. The typical yield of purified DNA from 10 ml of culture is 5-10 µg.

University of Kentucky (US)
Zero hunger
Openalex Percentile: Top 13%
Plant Disease Resistance and Genetics
1.75
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.

Isolation of Pyricularia and preparation of DNA for Illumina Sequencing v1 — Mark Farman · (2025) | TGRS Research Map | TGRS