Guanidinium-linked morpholino-siRNA chimera: synthesis, biophysical properties and <i>in vitro</i> activity
This study introduces guanidinium morpholino RNA (GMO-RNA), an RNA analogue with enhanced cell permeability, nuclease resistance and RNA interference activity. Self-penetrating GMO-RNA chimeras leverage guanidinium-mediated endocytic uptake across multiple cell lines. Thermal studies validated duplex stability, and Eg5-targeting GMO-siRNA achieved effective gene silencing in cervical cancer cells, demonstrating therapeutic potential.
Authors
- Atanu Ghosh (ORCID: https://orcid.org/0000-0001-9221-6025)
- Surajit Sinha (ORCID: https://orcid.org/0000-0001-8884-1194)
- Mandira Mukherjee (ORCID: https://orcid.org/0000-0003-4123-1377)
- Shalini Gupta (ORCID: https://orcid.org/0000-0002-3354-6152)
- Jayanta Kundu (ORCID: https://orcid.org/0000-0003-2669-8112)
- Subhamoy Pratihar
Institutions
- Indian Association for the Cultivation of Science (IN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc03543k
- Citations
- 1
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Science and Engineering Research Board