A highly sensitive fluorogenic assay for trypsin in urine: a simple diagnostic approach for pancreatic diseases
A water-soluble pyrene-malonate (PyM) probe enables ultrasensitive, non-invasive detection of urinary trypsin for early pancreatic disease screening. Protamine binding induces aggregation and excimer formation, while trypsin-mediated cleavage disassembles the aggregates and enables monomer fluorescence recovery. The system reliably detects trypsin in 10% human urine within physiological levels.
Authors
- Jae‐Min Lim (ORCID: https://orcid.org/0000-0002-7153-0194)
- H. S. Seong
- Seoung Ho Lee (ORCID: https://orcid.org/0000-0001-5647-1993)
Institutions
- Daegu University (KR)
Publication Details
- Journal
- Organic & Biomolecular Chemistry
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5ob01098e
- Primary Topic
- Pancreatitis Pathology and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.00