IN VITRO THYMIDINE PHOSPHORYLASE INHIBITORY AND ANTIGLYCATION POTENTIALS OF ANABASIS ARTICULATA HPLC PURIFIED FRACTIONS

Anabasis articulata, a medicinal plant renowned for its abundance of bioactive constituents, has been studied for its antioxidant, anticancer, and antidiabetic potentials.Our previous research highlighted its significant antioxidant, anti-inflammatory, and antidiabetic activities.Herein, we extend on thymidine phosphorylase (TP) inhibitory and antiglycation potential of HPLC-purified fractions of A. articulata stem and leaves methanolic extract.TP is a key enzyme in angiogenesis and cancer progression, whereas protein glycation is associated with metabolic illnesses, including diabetes and neurological disorders.The methanolic extract of A. articulata was subjected to silica gel chromatography, resulting in four pure fractions (A-1, D-1, B-1, C-1).Results showed that A-1 and B-1 exhibited highest TP inhibition (87.1 and 90.4%) with an IC of 1.9 0.9 and 3.1 0.2 mg/mL, respectively.Furthermore, the antiglycation test showed significant inhibitory effects, with fraction C-1 exhibiting the strongest antiglycation activity (89.7%, IC50 = 0.11 0.002 mg/mL), followed by A-1 (87.3%, IC50 = 2.68 0.098 mg/mL).The findings indicated that A. articulata exhibited considerable therapeutic effects in combating cancer-related angiogenesis and glycation-associated metabolic diseases such as diabetes.