Reducing binding site heterogeneity <i>via</i> truncation of the RTX domain enhances selectivity for rare earth element separation
Rare earth elements (REEs) are critical components of modern technologies but present a notoriously difficult separation challenge. Here, we report a rationally truncated variant of the calcium-binding RTX domain that improves REE selectivity by reducing binding site heterogeneity. The engineered scaffold, RTX(2), exhibits improved binding affinity, structure stability, pH-tunable elution, and enhanced chromatographic resolution, achieving over 200-fold selectivity within the REE series.
Authors
- Bradley S. Heater (ORCID: https://orcid.org/0000-0001-8851-4345)
- Katarzyna H. Kucharzyk (ORCID: https://orcid.org/0000-0001-8524-2989)
- Scott Banta (ORCID: https://orcid.org/0000-0001-7885-0150)
- Sameera Abeyrathna (ORCID: https://orcid.org/0000-0002-1346-6796)
- Farid F. Khoury (ORCID: https://orcid.org/0000-0002-1116-6941)
Institutions
- Battelle (US)
- Columbia University (US)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc03992d
- Citations
- 4
- Primary Topic
- Extraction and Separation Processes
- Type
- article
- Field-Weighted Citation Impact
- 2.85
Funders
- National Science Foundation
- Columbia University
- Defense Advanced Research Projects Agency