<i>KCNJ11</i> Gene Polymorphism and Its Association with Type 2 Diabetes Mellitus in Iraqi Population

Diabetes mellitus is a complicated metabolic condition marked by hyperglycemia, results in consequences that decrease quality of life and increasing mortality rates. (KCNJ11) Potassium inwardly-rectifying channel, subfamily J, member 11 gene mutations can result in susceptibility of T2DM. To verify the correlation between the KCNJ11 gene single nucleotide polymorphism and the incidence of type 2 diabetes mellitus in Iraqi society. A case-control study involved 150 patients with type 2 diabetes mellitus from the Diabetic Center at Al-Sader Teaching Hospital in Najaf City, Iraq, who were compared to 150 healthy control group. Both groups satisfied the inclusion requirements. Blood was taken from all participants, and standard methodologies were employed to assess fasting blood glucose (FBS), serum triglycerides, total cholesterol, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, and insulin levels. A HOMO-IR calculation was conducted. The KCNJ11 gene rs1800467 SNP was genotyped using the Polymerase Chain Reaction-Allele Specific (PCR-AS) approach. The GC genotype of the rs1800467 SNP showed significant variation between diabetic and control groups under genetic models (P<0.005). The minor allele frequency of rs1800467C was increased in T2DM patients compared to controls (OR = 5.58, 95% CI = 1.61 – 19.35, P = 0.0039). Single nucleotide polymorphism of the KCNJ11 gene (rs1800467) is significantly linked with susceptibility to type 2 diabetes mellitus in the Iraqi population.