Gastroprotective, Antioxidant, Anti-Inflammatory, and Toxicological Evaluation of Stem Bark Extracts of Vitellaria paradoxa and Parkia biglobosa

<b>Background/Objectives</b>: Oxidative stress is a pathophysiological factor that causes challenging issues in the treatment of several diseases, including gastric ulcer, inflammatory diseases, and adenocarcinomas. <i>V. paradoxa</i> and <i>P. biglobosa</i> are African plants whose parts are used for treating diseases, including gastrointestinal pathologies. This study aimed to characterize the gastroprotective, antioxidant, and anti-inflammatory activities of <i>V. paradoxa</i> and <i>P. biglobosa</i> stem bark extracts based on various solvents. <b>Methods</b>: The phytochemical screening and antioxidant evaluation were performed using radical scavenging (ABTS and DPPH) and reduction (FRAP and APM) methods. The anti-inflammatory activity was performed through an egg albumin denaturation model. The toxicological evaluation was performed on <i>Artemia salina</i> and female Wistar rat models, and the gastroprotective activity was carried out on an ethanolic-induced gastric ulcer rat model. <b>Results</b>: The results reported that <i>V. paradoxa</i> stem bark extracts contain catechin, epicatechin, ferulic acid, apigenin-7-gluc, and hesperidin, while <i>P. biglobosa</i> bark contains chlorogenic acid, catechin, caffeine, epicatechin, and cichoric acid. In the DPPH assay, the lowest scavenging capacities were 1.8 ± 0.21 mmol AAE/mg of dry extract (<i>V. paradoxa</i>, 97% ethanol) and 11.43 ± 0.208 mmol AAE/mg of dry extract (<i>P. biglobosa</i>, 50% ethanol). Similarly, for ABTS, the lowest scavenging capacities were 0.9726 ± 0.03952 mmol AAE/mg of dry extract (<i>V. paradoxa</i>, methanol with 1% HCl) and 1.3 mmol AAE/mg of dry extract (<i>P. biglobosa</i>, 97% ethanol), indicating strong antioxidant capacity. In the FRAP assay, both species reached a maximum reducing power of 2.39 mMol AAE/mg of dry extract (methanolic extract for <i>V. paradoxa</i>; methanol + 1% HCl for <i>P. biglobosa</i>). For APM, the 97% ethanolic extracts again showed the highest total antioxidant capacities: 31.78 ± 1.481 mMol AAE/mg (<i>V. paradoxa</i>) and 31.21 ± 0.852 mMol AAE/mg (<i>P. biglobosa</i>). The stem bark extracts of both <i>V. paradoxa</i> and <i>P. biglobosa</i> were revealed to be harmless in the <i>Artemia salina</i> as well as the rat model. The extracts of <i>V. paradoxa</i> as well as <i>P. biglobosa</i> exerted a stronger gastroprotective effect than omeprazole, a commonly used reference molecule. <b>Conclusions</b>: These extracts, rich in compounds exhibiting strong antioxidant, anti-inflammatory, and gastroprotective activities, surpassed omeprazole in ulcer protection in rat models. Their safety was confirmed in both <i>Artemia salina</i> and rodent assays. Future studies will explore their immunomodulatory, antiproliferative activities in vitro and in vivo and, specifically, the efficacy of isolated compounds in gastric adenocarcinoma models to assess these plants' anticancer potential and elucidate their underlying mechanisms.