Molecular profile of sensitization to dog allergens: diagnostics using the “AllergochipRF” platform

The aim of this study was to assess sensitization to molecular dog allergens Can f 1 – Can f 6 in patients with clinical manifestations of bronchial asthma, allergic rhinitis, conjunctivitis, and/or atopic dermatitis using the diagnostic platform “AllergochipRF.” Recombinant allergens Can f 1, Can f 2, Can f 4, Can f 5, and Can f 6 were expressed in a heterologous Escherichia coli-based system and purified by metal-chelate affinity chromatography. The secondary structure of the obtained proteins was characterized using circular dichroism spectroscopy. The study included 100 patients with allergy symptoms, of whom 64% were found to be sensitized to at least one of the Can f 1 – Can f 6 components. The results showed that the major allergens in dog allergy are the molecular allergens Can f 1, Can f 5, and Can f 6. These findings confirm the clinical relevance of molecular diagnostics for dog allergen sensitization and highlight the key role of these components in allergic response development.