qPCR assay for the Detection of Coralliophila galea v1

This real-time PCR assay is designed to detectCoralliphila galea(formerlyCoralliphila abbreviata) in DNA-extracted samples. Primers were developed to target the COX1 and 12S genes ofC. galea, using sequences obtained from the National Center for Biotechnology Information (NCBI). The assay was validated using tissue samples fromC. galeaand gut content samples fromPanulirus argusandPanulirus guttatusthat consumedC. galea. Additionally, validation included a primer efficiency test via serial dilutions, gene alignment via Sanger sequencing, and melt curves confirmed no primer dimers. This assay offers a potential tool for detectingC. galeain environmental DNA (eDNA) samples, enabling non-invasive monitoring in coral restoration sites.