Molecular profiling of soybean (Glycine max L.) genotypes using RAPD markers

DNA markers can provide an ideal al ternative method for evaluating genetic diversity in soybean germplasm. Soybean crop is nutritionally important legumes globally. The present investigation was carried out to study molecular and biochemical characterization of soybean (Glycin Max L) by using RAPD markers. PCR amplification was conducted using RAPD primers, specifically OPN-06, OPN-07, OPN-08, OPN-09 and OPN-10. The dendrogram revealed polymorphism at a high percentage. The findings indicated the highest genetic similarity (coefficient = 0.82) between AVT 23-89 and AVT23-92, while the lowest similarity 0.14 was observed between Phule Kimaya and Phule Kalyani in RAPD marker analysis. The genetic diversity uncovered in this study aligns with previous reports by Tidkeet al. (2018), which recorded a polymorphism percentage of 86.95%.

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Publication Details

Journal
International Journal of Advanced Biochemistry Research
Published
2025-01-01
DOI
https://doi.org/10.33545/26174693.2025.v9.i8f.5219
Primary Topic
Soybean genetics and cultivation
Type
article
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article

Molecular profiling of soybean (Glycine max L.) genotypes using RAPD markers

R. P. Singh, Kunvar Gyanendra Kumar, AS Pawar, MR Shelke
International Journal of Advanced Biochemistry Research
Soybean genetics and cultivation
article

Molecular profiling of soybean (Glycine max L.) genotypes using RAPD markers

R. P. Singh, Kunvar Gyanendra Kumar, AS Pawar, MR Shelke
article en

Abstract

DNA markers can provide an ideal al ternative method for evaluating genetic diversity in soybean germplasm. Soybean crop is nutritionally important legumes globally. The present investigation was carried out to study molecular and biochemical characterization of soybean (Glycin Max L) by using RAPD markers. PCR amplification was conducted using RAPD primers, specifically OPN-06, OPN-07, OPN-08, OPN-09 and OPN-10. The dendrogram revealed polymorphism at a high percentage. The findings indicated the highest genetic similarity (coefficient = 0.82) between AVT 23-89 and AVT23-92, while the lowest similarity 0.14 was observed between Phule Kimaya and Phule Kalyani in RAPD marker analysis. The genetic diversity uncovered in this study aligns with previous reports by Tidkeet al. (2018), which recorded a polymorphism percentage of 86.95%.

International Journal of Advanced Biochemistry ResearchVol. 9(8)
Zero hunger
Openalex Percentile: Top 85%
Soybean genetics and cultivation
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