A novel practice for Experimental Osteoporosis Model in Rats Mediated by Combining Phosphoric Acid Administration with Ovariectomy

Osteoporosis, a metabolic condition impacts bone health by affecting calcium metabolism. Aging and decreased estrogen levels lead to imbalanced endocrine function, increasing bone resorption and decreasing bone creation. Increased release of parathyroid hormone due to high phosphate intake results in hyperphosphatemia and the removal of calcium from the bone loading. The goal of this work was to develop a new method for quickly inducing osteoporosis in contrast to existing conventional techniques. Wistar female rats were exposed to ovariectomy and subsequently 10% phosphoric acid orally for 30 days to induce osteoporosis. Thirty adult Wistar female rats were equally divided into three groups: Naïve rats served as the control group (C), Bilateral ovariectomized rats (OVX group), and Sham group were exposed to the same surgical operation steps of OVX group without removing the ovaries. One week of recovery post-surgical operation, the OVX and Sham groups were given 10% phosphoric acid in drinking water for 30 days, following the removal of all surgical sutures and an inspection for any flaws. The outcomes demonstrated that the OVX and Sham groups had significantly lower bone density than the control group, despite that they had significantly higher levels of alkaline phosphates, osteocalcin, and parathyroid hormone. Interestingly, the OVX group's serum calcium levels were significantly lower than those of the control group. In conclusion, ovariectomy and 10% phosphoric acid administration enforced osteoporosis status via hyperphosphatemia, hyperparathyroidism, and disruption in the bone metabolism in female rats in relatively short-term induction, this practice is highly useful in investigations of bone metabolism and health.