Clearing of deparaffinized human brain tissue v1
This protocol is a detailed version of the deparaffinization protocol for human brain tissue applicable to different clearing protocols (SHORT and iDISCO). The method was optimized by the European Laboratory for Non-Linear Spectroscopy - University of Florence -Italy, and describes the process to deparaffinize human brain tissue to prepare them for optical clearing and fluorescent labelling, permitting the 3D reconstruction of the tissue at micrometer-resolution using fluorescence microscopy techniques.
Authors
- Danila Di Meo (ORCID: https://orcid.org/0000-0001-6545-0676)
- Irene Costantini (ORCID: https://orcid.org/0000-0002-8464-7324)
- Josephine Ramazzotti (ORCID: https://orcid.org/0009-0007-0341-9607)
- Aron Emmi
Institutions
- University of Padua (IT)
- Neuroscience Institute (IT)
- European University Institute (IT)
Publication Details
- Published
- 2025-03-04
- DOI
- https://doi.org/10.17504/protocols.io.kqdg3qmjev25/v1
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Laserlab-Europe
- European Commission
- Fondazione Cassa di Risparmio di Firenze
- National Institutes of Health
- HORIZON EUROPE Framework Programme