Evaluation of a new commercial assay for the measurement of tissue factor activity of extracellular vesicles isolated from human plasma
In this study, we found that the CY assay detected TF activity in EVs isolated from plasma. However, significant amounts of TFPI were present in the EV preparations using the CY EV isolation protocol, which inhibited TF activity, leading to lower apparent EV TF levels.
Authors
- Alan E. Mast (ORCID: https://orcid.org/0000-0003-3740-0318)
- Ana Teresa Azevedo Sachetto (ORCID: https://orcid.org/0000-0002-8234-1209)
- Nigel Mackman (ORCID: https://orcid.org/0000-0002-9170-7700)
Institutions
- University of North Carolina at Chapel Hill (US)
- Medical College of Wisconsin (US)
- Versiti Blood Center of Wisconsin (US)
Publication Details
- Journal
- Research and Practice in Thrombosis and Haemostasis
- Published
- 2025-08-01
- DOI
- https://doi.org/10.1016/j.rpth.2025.103007
- Citations
- 1
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- American Heart Association
- National Institutes of Health
- University of North Carolina at Chapel Hill