Generation of a human induced pluripotent stem cell line (LZUSHi003-A) from peripheral mononuclear cells of a patient carrying the c.3682delG mutation in the dystrophin gene
Duchenne muscular dystrophy (DMD) is characterized by progressive muscle degeneration and is caused by mutations in the dystrophin gene. Here, we used Sendai virus to generate a human induced pluripotent stem cell line, LZUSHi003-A, from the peripheral mononuclear cells of a 13-year-old male patient carrying the DMD gene mutation c.3682delG. The LZUSHi003-A cell line retained this mutation, while expressing pluripotency markers. Additionally, it demonstrated a normal karyotype and could differentiate into three embryonic germ layers.
Authors
- Y. Zhang (ORCID: https://orcid.org/0009-0004-7261-7955)
- Bei Gao
- Ashley G. Zhao
- Jing Yang (ORCID: https://orcid.org/0000-0002-8325-9688)
- Huijun Gao
- Zhiqiang Xiong (ORCID: https://orcid.org/0000-0002-7984-3662)
- Yang Jiao (ORCID: https://orcid.org/0000-0003-3957-3829)
- Jie Shi (ORCID: https://orcid.org/0000-0001-6072-7031)
- Ruifu Yang (ORCID: https://orcid.org/0000-0003-3219-7269)
- Lan Yang (ORCID: https://orcid.org/0009-0002-5573-2033)
Institutions
- Gansu Provincial Hospital (CN)
- First Affiliated Hospital of Xi'an Jiaotong University (CN)
- Lanzhou University Second Hospital (CN)
- Lanzhou University (CN)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2025-08-09
- DOI
- https://doi.org/10.1016/j.scr.2025.103800
- Primary Topic
- Muscle Physiology and Disorders
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Lanzhou University
- Natural Science Foundation of Gansu Province