Learning from fungicide resistance: Evolutionary insights to guide RNAi-based control of fungal crop pathogens
Crop protection against fungal pathogens is essential to prevent crop losses and maintain food security. Current crop protection relies heavily on chemical fungicides. However, rapid evolution of fungicide resistance, the constant appearance of new pathogens, and legislation against chemical pesticides due to concerns regarding their impact on human health and the environment, mean new crop protection strategies are urgently required. One elegant solution is double-stranded RNA-based crop protection, which aims to silence selected genes in the pathogen to reduce crop damage. This technology brings the promise of targeting specific genes, which could be chosen to maximise protection, minimize off-target effects and reduce the risk of resistance evolution. Here we discuss strategies for successful use of this novel technology based on lessons learned from fungicide resistance and recent discoveries in fungal evolution derived from genome-sequencing.
Authors
- Elena Baraldi
- Florian Härtig (ORCID: https://orcid.org/0000-0002-6255-9059)
- Gail M. Preston (ORCID: https://orcid.org/0000-0003-3882-4438)
- Joris Alkemade (ORCID: https://orcid.org/0000-0003-1477-2936)
- B. A. Fraaije
- Michael Seidl (ORCID: https://orcid.org/0000-0002-5218-2083)
- Jonatan Niño‐Sánchez (ORCID: https://orcid.org/0000-0002-0356-9871)
- Isabel Corkley (ORCID: https://orcid.org/0000-0003-3783-3180)
- Helen M. Cockerton (ORCID: https://orcid.org/0000-0002-7375-1804)
- Nichola J. Hawkins (ORCID: https://orcid.org/0000-0003-3389-0436)
- Timothy G. Barraclough (ORCID: https://orcid.org/0000-0002-8084-2640)
- B. Werner (ORCID: https://orcid.org/0000-0002-8787-6050)
- Alan G. Buddie (ORCID: https://orcid.org/0000-0002-7677-9300)
- Danna R. Gifford (ORCID: https://orcid.org/0000-0002-4990-8816)
- Joy Lyu (ORCID: https://orcid.org/0009-0001-5393-6609)
- Ester Gaya (ORCID: https://orcid.org/0000-0001-6404-4297)
- K. Kanyuka (ORCID: https://orcid.org/0000-0001-6324-4123)
- Pietro D. Spanu (ORCID: https://orcid.org/0000-0001-8928-6049)
- Aline Koch
Institutions
- CAB International (GB)
- National Institute of Agricultural Botany (GB)
- Utrecht University (NL)
- Justus-Liebig-Universität Gießen (DE)
- University of Kent (GB)
- Agricultural Development Advisory Service (United Kingdom) (GB)
- Rothamsted Research (GB)
- Royal Botanic Gardens, Kew (GB)
- Manchester Academic Health Science Centre (GB)
- Northeast Catholic College (US)
- University of Manchester (GB)
- University of Oxford (GB)
- Management Research Institute (US)
- Imperial College London (GB)
- University of Regensburg (DE)
- University of Reading (GB)
- Wageningen University & Research (NL)
- University of Bologna (IT)
Publication Details
- Journal
- Fungal Biology Reviews
- Published
- 2025-08-11
- DOI
- https://doi.org/10.1016/j.fbr.2025.100443
- Citations
- 6
- Primary Topic
- Plant Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 10.50